Code
MOG-MSIX-01AX
Test
Active-Compendium
81301x1
Clinical
Oncology,Pathology
Note: An additional patient sample from normal, non-tumor tissue is required for comparison testing in MSI Analysis. Please submit all specimens with one test requisition form.
Specimen requirements for normal tissue in order of preference are:
5 mL peripheral blood in EDTA tube OR
FFPE tissue slides or block containing only non-tumor tissue. Please label these as "normal tissue". OR
In cases where no alternative tissue is available, we can attempt to isolate non-tumor tissue from the tumor specimen submitted. Note "Use tumor sample for normal tissue" on requisition. See requirements below.
Specimen requirements for tumor tissue: FFPE tissue: Paraffin block is preferred.
Alternatively, send 1 H&E slide plus 5-10 unstained slides cut at 5 or more microns. Please use positively charged slides and 10% NBF fixative. Do not use zinc fixatives.
Specimen requirements for normal tissue in order of preference are:
5 mL peripheral blood in EDTA tube OR
FFPE tissue slides or block containing only non-tumor tissue. Please label these as "normal tissue". OR
In cases where no alternative tissue is available, we can attempt to isolate non-tumor tissue from the tumor specimen submitted. Note "Use tumor sample for normal tissue" on requisition. See requirements below.
Specimen requirements for tumor tissue: FFPE tissue: Paraffin block is preferred.
Alternatively, send 1 H&E slide plus 5-10 unstained slides cut at 5 or more microns. Please use positively charged slides and 10% NBF fixative. Do not use zinc fixatives.
Flag Active
True
Orderable Test Description
PCR and fragment analysis of paired normal and tumor tissue to determine microsatellite instability (MSI) at the standard five NCI-recommended loci. Positive results are reported as MSI-high (at least two markers are unstable) or MSI-low (one marker is unstable). Testing requires paired normal tissue or blood analysis and at least 20% tumor content in tumor samples submitted (after macro-dissection).
Orderable Turn Around Time
7 Days
Keywords
Extra Note
An additional patient sample from normal, non-tumor tissue is required for comparison testing. Please submit all specimens with one test requisition form.
Normal Tissue: 5 mL peripheral blood in EDTA tube OR FFPE tissue block containing non-tumor tissue labeled as "normal tissue". In cases where no alternative tissue is available, we can attempt to isolate non-tumor tissue from the tumor specimen submitted. Note "Use tumor sample for normal tissue" on requisition. NYS clients please provide date and time of Collection.
Orderable Biomarkers
NY Approved
True
Orderable Biomarkers JSON
{"Polymerase Chain Reaction (PCR)": {"Genomic Signatures": ["MSI"]}}
Keywords string
MSI, microsatellite instability, colorectal cancer, HNPCC, lynch syndrome, immunotherapy, immunooncology, immuno-oncology, immune-oncology Microsatellite Instability (MSI) by PCR
Title URL
microsatellite-instability-msi-by-pcr